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pcag gfp vector  (Addgene inc)


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    Structured Review

    Addgene inc pcag gfp vector
    Pcag Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+gfp+vector/pCAG-GFP+(Plasmid+%2311150)/pmc12637253-52-22-24
    Average 95 stars, based on 322 article reviews
    pcag gfp vector - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: Multiple Buster transposons from hAT superfamily displaying transposition activities in mammal cells
    Article Snippet: For the helper plasmid, transposase coding sequences (CDS) of AnBT , CrBT , HvBT , LcBT , and LlBT were humanized using the idtdna webserver ( https://sg.idtdna.com/pages/tools/codon-optimization-tool ), synthesized, and cloned into the pUC19 vector by GENEWIZ. .. After amplification by high-fidelity PCR with primers containing Eco RI and Not I sites, the CDSs were cloned into the commercially available pCAG-GFP vector (Addgene), which had been linearized by Eco RI and Not I digestion. ..

    Article Title: MKL/SRF and Bcl6 mutual transcriptional repression safeguards the fate and positioning of neocortical progenitor cells mediated by RhoA.
    Article Snippet: pCAG:GFP (Addgene) and pCAG:Nuclear Localization Signal (NLS)-GFP (F. Kubo, National Institute of Genetics) were used for co-electroporation. .. RhoA, MKL1, MKL2, and SRF sequences were amplified from cDNA and inserted into the pCAG:GFP vector or the pEBG vector for GFP or glutathione S-transferase (GST) fusion. pEBG was a gift from D. Baltimore (Addgene, plasmid no. 22227). ..

    Article Title: MKL/SRF and Bcl6 mutual transcriptional repression safeguards the fate and positioning of neocortical progenitor cells mediated by RhoA
    Article Snippet: pCAG:GFP (Addgene) and pCAG:Nuclear Localization Signal (NLS)-GFP (F. Kubo, National Institute of Genetics) were used for co-electroporation. .. RhoA, MKL1, MKL2, and SRF sequences were amplified from cDNA and inserted into the pCAG:GFP vector or the pEBG vector for GFP or glutathione S -transferase (GST) fusion. pEBG was a gift from D. Baltimore (Addgene, plasmid no. 22227). ..

    Polymerase Chain Reaction:

    Article Title: Multiple Buster transposons from hAT superfamily displaying transposition activities in mammal cells
    Article Snippet: For the helper plasmid, transposase coding sequences (CDS) of AnBT , CrBT , HvBT , LcBT , and LlBT were humanized using the idtdna webserver ( https://sg.idtdna.com/pages/tools/codon-optimization-tool ), synthesized, and cloned into the pUC19 vector by GENEWIZ. .. After amplification by high-fidelity PCR with primers containing Eco RI and Not I sites, the CDSs were cloned into the commercially available pCAG-GFP vector (Addgene), which had been linearized by Eco RI and Not I digestion. ..

    Clone Assay:

    Article Title: Multiple Buster transposons from hAT superfamily displaying transposition activities in mammal cells
    Article Snippet: For the helper plasmid, transposase coding sequences (CDS) of AnBT , CrBT , HvBT , LcBT , and LlBT were humanized using the idtdna webserver ( https://sg.idtdna.com/pages/tools/codon-optimization-tool ), synthesized, and cloned into the pUC19 vector by GENEWIZ. .. After amplification by high-fidelity PCR with primers containing Eco RI and Not I sites, the CDSs were cloned into the commercially available pCAG-GFP vector (Addgene), which had been linearized by Eco RI and Not I digestion. ..

    Article Title: Generation and characterization of Sema6aΔcyt conditional knockout mice
    Article Snippet: .. Full-length Sema6a cDNA or shortened Sema6a lacking the sequence coding for the intracellular domain (bp 2680-3766) were cloned into the pCAG-GFP vector (Addgene, Cat #11150) to obtain pCAG -promotor-driven expression of Sema6a and Sema6aΔcyt with a GFP signal sequence located upstream. ..

    Plasmid Preparation:

    Article Title: Multiple Buster transposons from hAT superfamily displaying transposition activities in mammal cells
    Article Snippet: For the helper plasmid, transposase coding sequences (CDS) of AnBT , CrBT , HvBT , LcBT , and LlBT were humanized using the idtdna webserver ( https://sg.idtdna.com/pages/tools/codon-optimization-tool ), synthesized, and cloned into the pUC19 vector by GENEWIZ. .. After amplification by high-fidelity PCR with primers containing Eco RI and Not I sites, the CDSs were cloned into the commercially available pCAG-GFP vector (Addgene), which had been linearized by Eco RI and Not I digestion. ..

    Article Title: Regeneration-specific promoter switching facilitates Mest expression in the mouse digit tip to modulate neutrophil response
    Article Snippet: .. To generate the Mest overexpression plasmid, we utilized the pCAG-GFP vector (pCAG-GFP was a gift from Connie Cepko; Addgene #11150). .. GFP was excised with EcoRI and NotI digestion and a synthesized Mest211 cDNA gBlock (Genbank: NM_008590; Integrated DNA Technologies) was inserted with NEBuilder HiFi DNA assembly (New England Biolabs).

    Article Title: Regeneration-specific promoter switching facilitates Mest expression in the mouse digit tip to modulate neutrophil response
    Article Snippet: .. To generate the Mest overexpression plasmid, we utilized the pCAG-GFP vector (pCAG-GFP was a gift from Connie Cepko; Addgene #11150). .. GFP was excised with EcoRI and NotI digestion and a synthesized Mest211 cDNA gBlock (Genbank: NM_008590 ; Integrated DNA Technologies) was inserted with NEBuilder HiFi DNA assembly (New England Biolabs).

    Article Title: MKL/SRF and Bcl6 mutual transcriptional repression safeguards the fate and positioning of neocortical progenitor cells mediated by RhoA.
    Article Snippet: pCAG:GFP (Addgene) and pCAG:Nuclear Localization Signal (NLS)-GFP (F. Kubo, National Institute of Genetics) were used for co-electroporation. .. RhoA, MKL1, MKL2, and SRF sequences were amplified from cDNA and inserted into the pCAG:GFP vector or the pEBG vector for GFP or glutathione S-transferase (GST) fusion. pEBG was a gift from D. Baltimore (Addgene, plasmid no. 22227). ..

    Article Title: MKL/SRF and Bcl6 mutual transcriptional repression safeguards the fate and positioning of neocortical progenitor cells mediated by RhoA
    Article Snippet: pCAG:GFP (Addgene) and pCAG:Nuclear Localization Signal (NLS)-GFP (F. Kubo, National Institute of Genetics) were used for co-electroporation. .. RhoA, MKL1, MKL2, and SRF sequences were amplified from cDNA and inserted into the pCAG:GFP vector or the pEBG vector for GFP or glutathione S -transferase (GST) fusion. pEBG was a gift from D. Baltimore (Addgene, plasmid no. 22227). ..

    Article Title: Hsd17b7 undergoes dynamic subcellular localization during Neuro2a differentiation
    Article Snippet: N-terminal and C-terminal tagged GFP plasmids with full length mouse Hydroxysteroid 17-β7 sequence were obtained from Sino Biological (MG51644-ACG, MG51644-ANG). .. The pCAG-GFP vector was acquired from Addgene (this plasmid was a gift from Connie Cepko, plasmid # 11150; http://n2t.net/addgene:11150 ). .. Hsd17b7 expression plasmids were reconstituted and transformed into OneShot TOP10 chemically competent E. coli (C404010, Invitrogen) using manufacturers protocols.

    Over Expression:

    Article Title: Regeneration-specific promoter switching facilitates Mest expression in the mouse digit tip to modulate neutrophil response
    Article Snippet: .. To generate the Mest overexpression plasmid, we utilized the pCAG-GFP vector (pCAG-GFP was a gift from Connie Cepko; Addgene #11150). .. GFP was excised with EcoRI and NotI digestion and a synthesized Mest211 cDNA gBlock (Genbank: NM_008590; Integrated DNA Technologies) was inserted with NEBuilder HiFi DNA assembly (New England Biolabs).

    Article Title: Regeneration-specific promoter switching facilitates Mest expression in the mouse digit tip to modulate neutrophil response
    Article Snippet: .. To generate the Mest overexpression plasmid, we utilized the pCAG-GFP vector (pCAG-GFP was a gift from Connie Cepko; Addgene #11150). .. GFP was excised with EcoRI and NotI digestion and a synthesized Mest211 cDNA gBlock (Genbank: NM_008590 ; Integrated DNA Technologies) was inserted with NEBuilder HiFi DNA assembly (New England Biolabs).

    Sequencing:

    Article Title: Generation and characterization of Sema6aΔcyt conditional knockout mice
    Article Snippet: .. Full-length Sema6a cDNA or shortened Sema6a lacking the sequence coding for the intracellular domain (bp 2680-3766) were cloned into the pCAG-GFP vector (Addgene, Cat #11150) to obtain pCAG -promotor-driven expression of Sema6a and Sema6aΔcyt with a GFP signal sequence located upstream. ..

    Expressing:

    Article Title: Generation and characterization of Sema6aΔcyt conditional knockout mice
    Article Snippet: .. Full-length Sema6a cDNA or shortened Sema6a lacking the sequence coding for the intracellular domain (bp 2680-3766) were cloned into the pCAG-GFP vector (Addgene, Cat #11150) to obtain pCAG -promotor-driven expression of Sema6a and Sema6aΔcyt with a GFP signal sequence located upstream. ..

    Article Title: Regulation of RNG105/caprin1 dynamics by pathogenic cytoplasmic FUS and TDP-43 in neuronal RNA granules modulates synaptic loss
    Article Snippet: .. The pCAG-GFP vector (Addgene, Watertown, MA) was used to construct all the plasmids for expressing RBPs in neurons. .. DNA fragments, obtained by PCR from each template using each primer set, were cloned into the EcoRI and NotI sites of the pCAG-GFP vector using the In-Fusion Cloning Kit (Takara Bio, Shiga, Japan).

    Construct:

    Article Title: Regulation of RNG105/caprin1 dynamics by pathogenic cytoplasmic FUS and TDP-43 in neuronal RNA granules modulates synaptic loss
    Article Snippet: .. The pCAG-GFP vector (Addgene, Watertown, MA) was used to construct all the plasmids for expressing RBPs in neurons. .. DNA fragments, obtained by PCR from each template using each primer set, were cloned into the EcoRI and NotI sites of the pCAG-GFP vector using the In-Fusion Cloning Kit (Takara Bio, Shiga, Japan).



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